By Oscar; Latner, A. L. Bodansky
Read Online or Download Advances in Clinical Chemistry (Vol. 19, 1977) PDF
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Additional resources for Advances in Clinical Chemistry (Vol. 19, 1977)
1"Cat 37°C or, with external cooling, at 30°C or 25°C. Monitoring wavelengths of 405 k 1 nm or 340 1 nm (bandwidths < 5 nm) isolated by a diffraction grating are selected as appropriate by the push button which programs for each type of assay. The second, reference cuvette of the double-beam photometer normally contains air; however, a dual-probe sample pick up is provided so that a sample or reagent blank can be used to compensate for possible errors introduced into some assays by the use of serum initiation.
M. Widdowson, J. R. Yenton and W. A. Hasson, at the 8th International Congress on Clinical Chemistry, Copenhagen, 1972. 2. , Fullerton, California 92634; Fig. 4. The absorbance signal is electronically differentiated to obtain its rate 34 D. W. MOSS FIG. 15. The Beckman System TR enzyme activity analyzer. ) of change with time, and the variation of the first differential with time is monitored by a second differentiating circuit (Fig. 9). , the reaction is following zeroorder kinetics. After the rapid fluctuations in absorbance due to reaction initiating and mixing have subsided, the second differential is monitored until a zero value lasting for 17 seconds is registered.
Within preset limits, the value of absorbance change per minute multiplied by an appropriate calibration factor is printed out as the catalytic concentration of the enzyme. If the expected relationship between Al and A? is not found, the reaction is followed for a further 20 seconds, then for 40 seconds more, and finally for 80 seconds. , monitoring being terminated as soon as the required degree of linearity has been detected. If no two successive comparisons are satisfactory, a result based on the last monitoring period is printed together with a "nonlinear" 50 D.